# loads the raw data, setting some default types for various columns load_raw <- function(filename) { read_csv(filename, col_types = cols( .default = col_character(), project_id = col_character(), id = col_character(), parameter_id = col_character(), age_in_days = col_integer(), date_of_experiment = col_datetime(format = ""), weight = col_double(), phenotyping_center_id = col_character(), production_center_id = col_character(), weight_date = col_datetime(format = ""), date_of_birth = col_datetime(format = ""), procedure_id = col_character(), pipeline_id = col_character(), biological_sample_id = col_character(), biological_model_id = col_character(), weight_days_old = col_integer(), datasource_id = col_character(), experiment_id = col_character(), data_point = col_double(), age_in_weeks = col_integer(), `_version_` = col_character() ) ) } # Apply some standard cleaning to the data clean_raw_data <- function(mydata) { mydata %>% # Fileter to IMPC source (recommened by Jeremey in email to Susi on 20 Aug 2018) filter(datasource_name == 'IMPC') %>% # standardise trait names mutate(parameter_name = tolower(parameter_name) ) %>% # remove extreme ages filter(age_in_days > 0 & age_in_days < 500) %>% # remove NAs filter(!is.na(data_point)) %>% # subset to reasonable set of variables # date_of_experiment: Jeremy suggested using as an indicator of batch-level effects select(production_center, strain_name, strain_accession_id, biological_sample_id, pipeline_stable_id, procedure_group, procedure_name, sex, date_of_experiment, age_in_days, weight, parameter_name, data_point) %>% arrange(production_center, biological_sample_id, age_in_days) } # subset data to select data for given parameter, and taking a single record per individual, choosing the record as close as possible to # age_center data_subset_parameter_individual_by_age <- function(mydata, parameter, age_min, age_center) { tmp <- mydata %>% filter(age_in_days >= age_min, parameter_name == parameter) %>% # take results for single individual closest to age_center mutate(age_diff = abs(age_center - age_in_days)) %>% group_by(biological_sample_id) %>% filter(age_diff == min(age_diff)) %>% select(-age_diff) # still some individuals with multiple records (because same individual appears under different procedures, so filter to one record) i <- match(unique(tmp$biological_sample_id), tmp$biological_sample_id) tmp[i, ] } #for loop across all traits (FZ) data_subset_parameterid_individual_by_age <- function(mydata, parameter, age_min, age_center) { tmp <- mydata %>% filter(age_in_days >= age_min, id == parameter) %>% # take results for single individual closest to age_center mutate(age_diff = abs(age_center - age_in_days)) %>% group_by(biological_sample_id) %>% filter(age_diff == min(age_diff)) %>% select(-age_diff) # still some individuals with multiple records (because same individual appears under different procedures, so filter to one record) i <- match(unique(tmp$biological_sample_id), tmp$biological_sample_id) tmp[i, ] }